This Biology Factsheet outlines the process of respiration and explains the factors influencing the rate of respiration, including temperature, substrate concentration, and different respiratory substrates. It describes why yeast is often used to investigate respiration and explains how a respirometer is used to investigate the effect of temperature on the rate of respiration. The Factsheet details ways to investigate aerobic respiration and respiration in anaerobic conditions. It also describes how to use a redox indicator (e.g. methylene blue or DCPIP) to investigate dehydrogenase activity and discusses the use of sensors, dataloggers, and software.

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Publication Date

January 2024


ISSN: 1351-5136

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438 Investigating Respiration

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