Be familiar with mixtures and separation techniques that are based on the physical properties of the components involved, e.g. melting-points, solubility.
Be familiar with qualitative analysis in identifying and confirming a sample substance through comparison with a known standard.
Be aware that forces of attraction act between different chemical species leading to greater or lesser affinity between particles.

After working through this Chemistry Factsheet, you will:

Be able to describe the use of thin-layer chromatography (TLC) to separate and identify components in a sample mixture by comparing R_f
values.
Be able to explain what is meant by stationary and mobile phases and how different affinities for the mobile and stationary phases leads to different R_f values or retention times.
Be able to correctly complete typical examination questions on thin-layer chromatography and gas chromatography (GC).

Download Type

.PDF (pdf) 531.359 KB

Publication Date

January 2023

ISSN / ISBN

ISSN: 1351-5136

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348 Using Chromatography in the Laboratory

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